Journal: Journal of Clinical Laboratory Analysis

Article Title: Circulating cell‐free circRNA panel predicted tumorigenesis and development of colorectal cancer

doi: 10.1002/jcla.24431

Figure Lengend Snippet: Circulating circRNAs expression landscape of in CRC, CRA, and healthy control samples. (A and B) Cluster analysis of differentially expressed circRNA in each group. (C) Volcano blot presented differentially expressed circRNA. (D) Intersection matching analysis of CRA group and healthy control compared with CRC group. CRA, colorectal adenomas; CRC, colorectal cancer

Article Snippet: The circRNA microarray was employed by using Arraystar Circular RNA Microarray Version 2.0.

Techniques: Expressing, Control

Journal: Journal of Clinical Laboratory Analysis

Article Title: Circulating cell‐free circRNA panel predicted tumorigenesis and development of colorectal cancer

doi: 10.1002/jcla.24431

Figure Lengend Snippet: Relative expression of circRNA in training set. The eight circRNAs were examined in 20 paired samples of three group. Data was presented as mean ± SD, **indicated p < 0.01. *indicated p < 0.05, n.s, indicated no significance

Article Snippet: The circRNA microarray was employed by using Arraystar Circular RNA Microarray Version 2.0.

Techniques: Expressing

Journal: Journal of Clinical Laboratory Analysis

Article Title: Circulating cell‐free circRNA panel predicted tumorigenesis and development of colorectal cancer

doi: 10.1002/jcla.24431

Figure Lengend Snippet: Relative expression of candidate circRNA in independent cohort. The eight circRNAs were examined in 80 paired plasma samples from healthy controls, CRA patients and CRC patients. **indicated p < 0.01. *indicated p < 0.05, n.s, indicated no significance

Article Snippet: The circRNA microarray was employed by using Arraystar Circular RNA Microarray Version 2.0.

Techniques: Expressing, Clinical Proteomics

Journal: Journal of Clinical Laboratory Analysis

Article Title: Circulating cell‐free circRNA panel predicted tumorigenesis and development of colorectal cancer

doi: 10.1002/jcla.24431

Figure Lengend Snippet: CircRNA panel predicted CRC from healthy controls. (A) Diagnostic efficacy of three circRNA panel as diagnostic marker for colorectal cancer in healthy population in training sets. (B) Diagnostic efficacy of three circRNA panel as biomarker of colorectal cancer in healthy population in validation set

Article Snippet: The circRNA microarray was employed by using Arraystar Circular RNA Microarray Version 2.0.

Techniques: Diagnostic Assay, Marker, Biomarker Discovery

Journal: Journal of Clinical Laboratory Analysis

Article Title: Circulating cell‐free circRNA panel predicted tumorigenesis and development of colorectal cancer

doi: 10.1002/jcla.24431

Figure Lengend Snippet: CircRNA panel predicted CRC from CRA. (A) Diagnostic efficacy of three circRNA panel as diagnostic marker for colorectal cancer in CRA patients in training sets. (B) Diagnostic efficacy of three circRNA panel as biomarker of colorectal cancer in CRA patients in validation set

Article Snippet: The circRNA microarray was employed by using Arraystar Circular RNA Microarray Version 2.0.

Techniques: Diagnostic Assay, Marker, Biomarker Discovery

Journal: Frontiers in Cell and Developmental Biology

Article Title: Differential CircRNA Expression Signatures May Serve as Potential Novel Biomarkers in Prostate Cancer

doi: 10.3389/fcell.2021.605686

Figure Lengend Snippet: Clustering heatmap of microarray data showing differential expression of circRNAs between malignant, benign and normal cell lines. Unsupervised clustering (euclidean distance measure and the “average” agglomeration method) was used for analysis ( n = 3). circRNAs were more likely to be down-regulated in normal and benign cell lines compared to malignant cells.

Article Snippet: The human circular RNA microarray (Arraystar) version 2.0 covers 13,617 previously discovered human circRNAs.

Techniques: Microarray, Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Differential CircRNA Expression Signatures May Serve as Potential Novel Biomarkers in Prostate Cancer

doi: 10.3389/fcell.2021.605686

Figure Lengend Snippet: Top 10 down-regulated circRNAs in PCa (malignant vs. normal/benign cell lines)*.

Article Snippet: The human circular RNA microarray (Arraystar) version 2.0 covers 13,617 previously discovered human circRNAs.

Techniques:

Journal: Frontiers in Cell and Developmental Biology

Article Title: Differential CircRNA Expression Signatures May Serve as Potential Novel Biomarkers in Prostate Cancer

doi: 10.3389/fcell.2021.605686

Figure Lengend Snippet: Clustering heatmap showing differential expression of circRNAs between AR dependent cells LNCaP, 22Rv1 and VCaP (hormone sensitive) and AR independent cells DU145 and PC-3 (castration resistant). Unsupervised clustering (euclidean distance measure and the “average” agglomeration method) was used for analysis ( n = 3).

Article Snippet: The human circular RNA microarray (Arraystar) version 2.0 covers 13,617 previously discovered human circRNAs.

Techniques: Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Differential CircRNA Expression Signatures May Serve as Potential Novel Biomarkers in Prostate Cancer

doi: 10.3389/fcell.2021.605686

Figure Lengend Snippet: Top 10 up-regulated circRNAs in androgen dependent vs. independent cell lines*.

Article Snippet: The human circular RNA microarray (Arraystar) version 2.0 covers 13,617 previously discovered human circRNAs.

Techniques:

Journal: Frontiers in Cell and Developmental Biology

Article Title: Differential CircRNA Expression Signatures May Serve as Potential Novel Biomarkers in Prostate Cancer

doi: 10.3389/fcell.2021.605686

Figure Lengend Snippet: Top 10 down-regulated circRNAs in androgen dependent vs. independent cell lines*.

Article Snippet: The human circular RNA microarray (Arraystar) version 2.0 covers 13,617 previously discovered human circRNAs.

Techniques:

Journal: Molecular Medicine Reports

Article Title: CircRNA expression profiles in human visceral preadipocytes and adipocytes

doi: 10.3892/mmr.2019.10886

Figure Lengend Snippet: Comparison of circRNA expression profiles between HPA-v and adipocytes. (A) Box plots revealed the distribution of circRNAs in the six samples after normalization. (B) Volcano plots revealed the differentially expressed circRNAs. Green and red dots represent significantly down- and upregulated circRNAs in adipocytes compared with HPA-v, respectively (fold change ≥5.0, P<0.01). (C) Hierarchical clustering was performed to reveal the differentially expressed circRNAs between HPA-v and adipocytes. (D) Expression patterns of select differentially expressed circRNAs in HPA-v and adipocytes were determined by qPCR. (E) The heatmap revealed the selected differentially expressed circRNAs in HPA-v and adipocytes. *P<0.05. circRNA, circular RNA; HPA-v, human preadipocytes from visceral fat tissue; AD, adipocytes; n.d., not detected.

Article Snippet: To investigate whether circRNAs are associated with lipid deposition, a human circRNA microarray (version 2.0; Agilent Technologies, Inc.) was used to assess circRNA expression profiles in HPA-v and adipocytes.

Techniques: Expressing

Journal: Molecular Medicine Reports

Article Title: CircRNA expression profiles in human visceral preadipocytes and adipocytes

doi: 10.3892/mmr.2019.10886

Figure Lengend Snippet: Top 10 up- and downregulated circRNAs in adipocytes.

Article Snippet: To investigate whether circRNAs are associated with lipid deposition, a human circRNA microarray (version 2.0; Agilent Technologies, Inc.) was used to assess circRNA expression profiles in HPA-v and adipocytes.

Techniques:

Journal: Molecular Medicine Reports

Article Title: CircRNA expression profiles in human visceral preadipocytes and adipocytes

doi: 10.3892/mmr.2019.10886

Figure Lengend Snippet: The top 15 significantly enriched pathways associated with the differentially expressed circRNA parental genes according to KEGG analysis. circRNAs, circular RNAs; KEGG, Kyoto Encyclopedia of Genes and Genomes.

Article Snippet: To investigate whether circRNAs are associated with lipid deposition, a human circRNA microarray (version 2.0; Agilent Technologies, Inc.) was used to assess circRNA expression profiles in HPA-v and adipocytes.

Techniques:

Journal: Molecular Medicine Reports

Article Title: CircRNA expression profiles in human visceral preadipocytes and adipocytes

doi: 10.3892/mmr.2019.10886

Figure Lengend Snippet: miRNAs with >2 miRNA response elements targeting the top 10 upregulated and downregulated circRNAs.

Article Snippet: To investigate whether circRNAs are associated with lipid deposition, a human circRNA microarray (version 2.0; Agilent Technologies, Inc.) was used to assess circRNA expression profiles in HPA-v and adipocytes.

Techniques:

Journal: Cell Death Discovery

Article Title: CircRNA circ-NNT mediates myocardial ischemia/reperfusion injury through activating pyroptosis by sponging miR-33a-5p and regulating USP46 expression

doi: 10.1038/s41420-021-00706-7

Figure Lengend Snippet: A The heat map shows the 20 differently expressed circRNAs, which were analyzed by circRNAs Arraystar Chip. B The relative mRNA expression of 10 down-regulated circRNAs were detected by qRT-PCR ( n = 10/group). C The relative mRNA expression of 10 up-regulated circRNAs were detected by qRT-PCR ( n = 10/group). D Scheme illustrating the production of circ-NNT. Circ-NNT was formed by back splicing at exon 17 of the NNT gene. E The relative mRNA expression of circ-NNT was shown in a scatter plots (the top upregulated circRNAs), which was measured by qRT-PCR ( n = 10/group). F The gel electrophoresis validated the existence of circ-NNT. G Circ-NNT and NNT mRNA levels in cardiomyocytes with or without RNase R treatment were measured by qRT-PCR. ns: no significant, *** P < 0.0001.

Article Snippet: A The heat map shows the 20 differently expressed circRNAs, which were analyzed by circRNAs Arraystar Chip.

Techniques: Expressing, Quantitative RT-PCR, Nucleic Acid Electrophoresis